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Santa Cruz Biotechnology cdk6
Cdk6, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 92/100, based on 24 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/cdk6/Cdk4+siRNA/us12595483-452-43-50
Average 92 stars, based on 24 article reviews
cdk6 - by Bioz Stars, 2026-10
92/100 stars

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Related Articles

other:

Article Title: Oncogenic and immunological roles of LAMP5 across cancers and its potential utility in bladder cancer.
Article Snippet: Zhenni Lin and Kun Zhao contributed equally to this work.. Abstract Lysosomal associated membrane protein family member 5 (LAMP5), a recently identified member of the LAMP family, has been associated with poor prognosis in multiple cancer types; however, its precise oncogenic mechanisms remain unclear.. This study systematically investigated the oncogenic and immunological functions of LAMP5 using multiple datasets.

Article Title: Eugenol as a Novel Neuroblastoma Therapeutic Identified by an Online Prognostic Assessment Platform
Article Snippet: CDK4 (#sc-56277, Dilution ratio 1:1000) and CDK6 (#sc-53638, Dilution ratio 1:1000) antibodies were purchased from Santa Cruz (Dallas, TX, USA).

Cell Culture:

Article Title: Treatment of tumors with miRNA targeting CDK4/CDK6
Article Snippet: Throughout these examples, molecular cloning reactions, and other standard recombinant DNA techniques, were carried out according to methods described in Maniatis et al., Molecular Cloning—A Laboratory Manual, 2nd ed., Cold Spring Harbor Press (1989), using commercially available reagents, except where otherwise noted. .. Cell culture and reagents: All colorectal, pancreatic and melanoma cell lines were obtained from American Type Culture Collection and maintained in the recommended media. miRNA mimics for hsa-miR-6883-5p, hsa-miR-149*, and hsa-miR-206 (HMI2616, HMI0241 and HMI0364) were purchased from Sigma-Aldrich. siRNA for CDK4 and CDK6 (sc-29261 and sc-29264) were purchased from Santa Cruz Biotechnology. .. The CDK4-Luciferase construct was obtained from Origene Technologies.

Bicinchoninic Acid Protein Assay:

Article Title: Lactylation-driven KRT19 promotes non-small cell lung cancer progression by suppressing cellular senescence
Article Snippet: Tissues and cells were lysed with RIPA buffer (G2002, Servicebio) containing protease inhibitor (G2007, Servicebio) and 1 mM PMSF (G2008, Servicebio). .. Protein extracts were quantified with the BCA Protein Assay Kit (WB6501, NCM Biotech), and immunoblotted with primary antibodies against H3K18la (PTM-1406RM, PTM BIO), Histone H3 (17168-1-AP, Proteintech), KRT19 (10712-1-AP, Proteintech), MYH9 (11128-1-AP, Proteintech), p53 (10442-1-AP, Proteintech), p21 (10355-1-AP, Proteintech), CDK1 (ab133327, Abcam), CDK6 (CY5835, Abways), Cyclin D1 (CY5404, Abways), LDHA (CY5348, Abways), LDHB (sc-100775, Santa Cruz), Ubiquitin (sc-166553, Santa Cruz), α-Tubulin (66031-1-Ig, Proteintech), Flag tag (66008-4-Ig, Proteintech), HA tag (51064-2-AP, Proteintech), Myc tag (AB0001, Abways) and appropriated secondary horseradish peroxidase-labeled antibodies. .. Indicated protein was detected with Enhanced chemiluminescence (SQ201, Epizyme) and imaged on Azure Biosystems C300.

Article Title: Lactylation-driven KRT19 promotes non-small cell lung cancer progression by suppressing cellular senescence.
Article Snippet: Tissues and cells were lysed with RIPA buffer (G2002, Servicebio) containing protease inhibitor (G2007, Servicebio) and 1 mM PMSF (G2008, Servicebio). .. Protein extracts were quantified with the BCA Protein Assay Kit (WB6501, NCM Biotech), AR TIC LE IN PR ES S and immunoblotted with primary antibodies against H3K18la (PTM-1406RM, PTM BIO), Histone H3 (17168-1-AP, Proteintech), KRT19 (10712-1-AP, Proteintech), MYH9 (11128-1-AP, Proteintech), p53 (10442-1-AP, Proteintech), p21 (10355-1-AP, Proteintech), CDK1 (ab133327, Abcam), CDK6 (CY5835, Abways), Cyclin D1 (CY5404, Abways), LDHA (CY5348, Abways), LDHB (sc-100775, Santa Cruz), Ubiquitin (sc-166553, Santa Cruz), α-Tubulin (66031-1-Ig, Proteintech), Flag tag (66008-4-Ig, Proteintech), HA tag (51064-2-AP, Proteintech), Myc tag (AB0001, Abways) and appropriated secondary horseradish peroxidase-labeled antibodies. .. Indicated protein was detected with Enhanced chemiluminescence (SQ201, Epizyme) and imaged on Azure Biosystems C300.

Ubiquitin Proteomics:

Article Title: Lactylation-driven KRT19 promotes non-small cell lung cancer progression by suppressing cellular senescence
Article Snippet: Tissues and cells were lysed with RIPA buffer (G2002, Servicebio) containing protease inhibitor (G2007, Servicebio) and 1 mM PMSF (G2008, Servicebio). .. Protein extracts were quantified with the BCA Protein Assay Kit (WB6501, NCM Biotech), and immunoblotted with primary antibodies against H3K18la (PTM-1406RM, PTM BIO), Histone H3 (17168-1-AP, Proteintech), KRT19 (10712-1-AP, Proteintech), MYH9 (11128-1-AP, Proteintech), p53 (10442-1-AP, Proteintech), p21 (10355-1-AP, Proteintech), CDK1 (ab133327, Abcam), CDK6 (CY5835, Abways), Cyclin D1 (CY5404, Abways), LDHA (CY5348, Abways), LDHB (sc-100775, Santa Cruz), Ubiquitin (sc-166553, Santa Cruz), α-Tubulin (66031-1-Ig, Proteintech), Flag tag (66008-4-Ig, Proteintech), HA tag (51064-2-AP, Proteintech), Myc tag (AB0001, Abways) and appropriated secondary horseradish peroxidase-labeled antibodies. .. Indicated protein was detected with Enhanced chemiluminescence (SQ201, Epizyme) and imaged on Azure Biosystems C300.

Article Title: Lactylation-driven KRT19 promotes non-small cell lung cancer progression by suppressing cellular senescence.
Article Snippet: Tissues and cells were lysed with RIPA buffer (G2002, Servicebio) containing protease inhibitor (G2007, Servicebio) and 1 mM PMSF (G2008, Servicebio). .. Protein extracts were quantified with the BCA Protein Assay Kit (WB6501, NCM Biotech), AR TIC LE IN PR ES S and immunoblotted with primary antibodies against H3K18la (PTM-1406RM, PTM BIO), Histone H3 (17168-1-AP, Proteintech), KRT19 (10712-1-AP, Proteintech), MYH9 (11128-1-AP, Proteintech), p53 (10442-1-AP, Proteintech), p21 (10355-1-AP, Proteintech), CDK1 (ab133327, Abcam), CDK6 (CY5835, Abways), Cyclin D1 (CY5404, Abways), LDHA (CY5348, Abways), LDHB (sc-100775, Santa Cruz), Ubiquitin (sc-166553, Santa Cruz), α-Tubulin (66031-1-Ig, Proteintech), Flag tag (66008-4-Ig, Proteintech), HA tag (51064-2-AP, Proteintech), Myc tag (AB0001, Abways) and appropriated secondary horseradish peroxidase-labeled antibodies. .. Indicated protein was detected with Enhanced chemiluminescence (SQ201, Epizyme) and imaged on Azure Biosystems C300.

FLAG-tag:

Article Title: Lactylation-driven KRT19 promotes non-small cell lung cancer progression by suppressing cellular senescence
Article Snippet: Tissues and cells were lysed with RIPA buffer (G2002, Servicebio) containing protease inhibitor (G2007, Servicebio) and 1 mM PMSF (G2008, Servicebio). .. Protein extracts were quantified with the BCA Protein Assay Kit (WB6501, NCM Biotech), and immunoblotted with primary antibodies against H3K18la (PTM-1406RM, PTM BIO), Histone H3 (17168-1-AP, Proteintech), KRT19 (10712-1-AP, Proteintech), MYH9 (11128-1-AP, Proteintech), p53 (10442-1-AP, Proteintech), p21 (10355-1-AP, Proteintech), CDK1 (ab133327, Abcam), CDK6 (CY5835, Abways), Cyclin D1 (CY5404, Abways), LDHA (CY5348, Abways), LDHB (sc-100775, Santa Cruz), Ubiquitin (sc-166553, Santa Cruz), α-Tubulin (66031-1-Ig, Proteintech), Flag tag (66008-4-Ig, Proteintech), HA tag (51064-2-AP, Proteintech), Myc tag (AB0001, Abways) and appropriated secondary horseradish peroxidase-labeled antibodies. .. Indicated protein was detected with Enhanced chemiluminescence (SQ201, Epizyme) and imaged on Azure Biosystems C300.

Article Title: Lactylation-driven KRT19 promotes non-small cell lung cancer progression by suppressing cellular senescence.
Article Snippet: Tissues and cells were lysed with RIPA buffer (G2002, Servicebio) containing protease inhibitor (G2007, Servicebio) and 1 mM PMSF (G2008, Servicebio). .. Protein extracts were quantified with the BCA Protein Assay Kit (WB6501, NCM Biotech), AR TIC LE IN PR ES S and immunoblotted with primary antibodies against H3K18la (PTM-1406RM, PTM BIO), Histone H3 (17168-1-AP, Proteintech), KRT19 (10712-1-AP, Proteintech), MYH9 (11128-1-AP, Proteintech), p53 (10442-1-AP, Proteintech), p21 (10355-1-AP, Proteintech), CDK1 (ab133327, Abcam), CDK6 (CY5835, Abways), Cyclin D1 (CY5404, Abways), LDHA (CY5348, Abways), LDHB (sc-100775, Santa Cruz), Ubiquitin (sc-166553, Santa Cruz), α-Tubulin (66031-1-Ig, Proteintech), Flag tag (66008-4-Ig, Proteintech), HA tag (51064-2-AP, Proteintech), Myc tag (AB0001, Abways) and appropriated secondary horseradish peroxidase-labeled antibodies. .. Indicated protein was detected with Enhanced chemiluminescence (SQ201, Epizyme) and imaged on Azure Biosystems C300.

Blocking Assay:

Article Title: KRAS mutated lung adenocarcinoma responds to pan-ERBB and Aurora kinase inhibitors
Article Snippet: Lysates were loaded on 8–12% polyacrylamide gels (as appropriate), separated using an SDS-containing running buffer, and transferred to nitrocellulose membranes (Invitrogen) by semi-dry blotting. .. After blocking (5% BSA or milk in 1x TBS-T for 1 h at room temperature), membranes were probed with primary antibodies against phospho-EGFR (Y1068, Cell Signaling #2234), EGFR (Cell Signaling #4267), phospho-ERK1/2 (T202/Y204, Cell Signaling #4376), ERK1/2 (Cell Signaling #4695), phospho-Histone H3 (Ser10, Cell Signaling #9701), Histone H3 (Cell Signaling #4499), Cyclin B1 (Santa Cruz, sc-7393), phospho-Aurora A /Aurora B/ Aurora C (T288 /T232 /T198, Cell Signaling #2914), Aurora A (Santa Cruz sc-56881), Aurora B (Cell Signaling #3094), phospho-STAT3 (Y705, Cell Signaling #9131), STAT3 (Cell Signaling #9139), phospho-TBK1 (S172, Cell Signaling #5483), TBK1 (Cell Signaling #3504), phospho-p38 (T180/Y182, Cell Signaling #4511), p38 (Cell Signaling #8690), CDK6 (Santa Cruz sc-56282), CDK4 (Santa Cruz sc-23896), Cyclin D1 (Santa Cruz sc-8396), RB (Santa Cruz sc-102), phospho-RB (S807/811, Cell Signaling 9308), phospho-AKT (S473, Cell Signaling #9271) and AKT (Cell Signaling #9272). .. HSC70 (Santa Cruz sc-7298) and ß-Actin (Santa Cruz sc-47778) were used as a loading control.



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